Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8.
FEBS Lett
; 318(1): 4-6, 1993 Feb 22.
Article
em En
| MEDLINE
| ID: mdl-8436222
ABSTRACT
We have investigated whether a spliceosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different beta-galactosidase-PRP8 fusion proteins. The < 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Plantas Tóxicas
/
Saccharomyces cerevisiae
/
Nicotiana
/
Proteínas Fúngicas
/
Spliceossomos
/
Proteínas de Saccharomyces cerevisiae
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article