A rapid GLC method for the direct analysis of plasma medium chain fatty acids.
Clin Chim Acta
; 214(1): 21-30, 1993 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-8453775
ABSTRACT
A new, rapid, gas-liquid-chromatographic (GLC) method for the direct determination of plasma medium-chain fatty acids (MCFA) (from C8 to C12) which does not require derivatization procedures is described. Analysis of underivatized MCFA by GLC showed a detection limit ranging from 0.50 to 0.217 microgram depending on chain length the longer the chain, the higher the detection limit. Compared with the HPLC and GLC methods currently described in the literature, this GLC procedure appears to be easy, adequately sensitive, accurate and well reproducible for those MCFA, like n-octanoic and n-decanoic acids, present in plasma after p.o. or i.v. MCT administration and it requires a short time (about 30 min) for complete processing.
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia Gasosa
/
Ácidos Graxos
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article