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The development and validation of a simple antigen detection ELISA for Plasmodium falciparum malaria.
Taylor, D W; Voller, A.
Afiliação
  • Taylor DW; Department of Biology, Georgetown University, Washington, DC 20057-1028.
Trans R Soc Trop Med Hyg ; 87(1): 29-31, 1993.
Article em En | MEDLINE | ID: mdl-8465387
A double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) is described for the detection of Plasmodium falciparum antigen. The test is based on an immunoglobulin (Ig) M capture monoclonal antibody on the solid phase and an IgG monoclonal antibody conjugated to peroxidase. The simple test takes about 2.5 h to complete and, because it uses whole blood with no prior treatment, it is possible to process batches of 50-100 samples simultaneously. The test is specific to P.falciparum and has a sensitivity close to that usually achieved with Giemsa-stained blood films. The reagents employed are stable at refrigerator temperatures for over 6 months, and as the test is compatible with human immunodeficiency virus and hepatitis B surface antigen ELISAs it could be suitable for blood transfusion screening.
Assuntos
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Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Malária Falciparum / Anticorpos Monoclonais / Antígenos de Protozoários Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 1993 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Malária Falciparum / Anticorpos Monoclonais / Antígenos de Protozoários Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 1993 Tipo de documento: Article