Quantitative analysis of protein and non-protein amino acids by gas-liquid chromatography.

ABSTRACT

The quantitative analysis of protein and non-protein amino acids by gas-liquid chromatography is described. The amino acids are determined as their n-butyl N-trifluoroacetyl esters on an OV-17 (methylphenyl silicone polymer) column. The relative molar responses of 16 protein and 30 non-protein amino acids are reported.