Quantification of DNA damage and repair in amino acid auxotrophs and UV-sensitive mutants of Aspergillus nidulans using an ELISA.
FEBS Lett
; 377(2): 118-22, 1995 Dec 18.
Article
em En
| MEDLINE
| ID: mdl-8543032
ABSTRACT
An ELISA used to investigate DNA repair in mammalian cells has been adapted to investigate mutagen-induced DNA damage and repair in protoplasts of Aspergillus nidulans. The assay shows a reduced rate of repair of DNA damage in methionine and arginine auxotrophs (methG and argB), which were shown previously to be hypersensitive to UV radiation and chemical mutagens. The assay also showed a considerably reduced ability to repair mutagen-induced damage in the uv-sensitive mutants uvsB and uvsH. The increased sensitivity of amino acids auxotrophs to mutagens is, therefore, correlated with a reduced capacity to repair mutagen-induced DNA damage.
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Base de dados:
MEDLINE
Assunto principal:
Arginina
/
Aspergillus nidulans
/
Dano ao DNA
/
Ensaio de Imunoadsorção Enzimática
/
Reparo do DNA
/
Metionina
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article