Interleukin-1 beta induces expression of cyclooxygenase-2 mRNA in human gingival fibroblasts.
Inflammation
; 19(5): 549-60, 1995 Oct.
Article
em En
| MEDLINE
| ID: mdl-8543370
ABSTRACT
The effect of interleukin-1 beta (IL-1 beta) on the expression of cyclooxygenase-1 and -2 (COX-1 and COX-2) mRNA and its relation to prostaglandin E2 (PGE2) biosynthesis in human gingival fibroblasts was studied. IL-1 beta increased levels of mRNA for COX-2 whereas the COX-1 mRNA level was unaffected. The increased COX-2 mRNA levels were accompanied by enhanced PGE2 formation. The phorbol, 12-myristate 13-acetate (PMA), known to stimulate protein kinase C (PKC), also induced expression of COX-2 mRNA. When gingival fibroblasts were treated simultaneously with IL-1 beta and PMA, the cytokine IL-1 beta synergistically increased levels of COX-2 mRNA, accompanied by a corresponding increase in PGE2 biosynthesis. The anti-inflammatory steroid, dexamethasone (DEX) abolished the enhanced expression of COX-2 mRNA as well as PGE2 formation induced by IL-1 beta, PMA or the combination of IL-1 beta and PMA. The study indicates that the IL-1 beta induced PGE2 formation is mediated by an enhanced gene expression of COX-2 in gingival fibroblasts suggesting that the enzyme COX-2 may play an important role in the regulation of prostanoid formation at inflammatory lesions in gingival tissue.
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Interleucina-1
/
Prostaglandina-Endoperóxido Sintases
/
Gengiva
/
Isoenzimas
Limite:
Child
/
Humans
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article