Analysis of alloreactive helper T lymphocyte precursor frequencies. Influence of interleukin-2 produced by the stimulating cells.
J Immunol Methods
; 189(1): 65-72, 1996 Jan 16.
Article
em En
| MEDLINE
| ID: mdl-8576581
ABSTRACT
In the determination of alloreactive helper T lymphocyte precursor frequencies (HTLpf) by limiting dilution analysis (LDA), peripheral blood mononuclear cells (PBMC) are used as stimulating cells. Interleukin-2 (IL-2) production by stimulating T cells constitute a source of error in these assays. We found that 100-150 Gy of gamma irradiation was required to abrogate IL-2 production by stimulating PBMC. This dose of irradiation, however, greatly reduced their allostimulatory capability. Here we describe how non-irradiated PBMC, immunomagnetically depleted of T cells and thus of IL-2 producing cells, can be used as stimulators in assays to determine alloresponsive HTLpf.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Ativação Linfocitária
/
Interleucina-2
/
Linfócitos T Auxiliares-Indutores
Limite:
Humans
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article