Stability study of Rhodobacter capsulatus ferrocytochrome c2 wild-type and site-directed mutants using hydrogen/deuterium exchange monitored by electrospray ionization mass spectrometry.
FEBS Lett
; 380(1-2): 44-8, 1996 Feb 12.
Article
em En
| MEDLINE
| ID: mdl-8603744
ABSTRACT
To estimate the stability of Rhodobacter capsulatus ferrocytochrome c2 wild-type and site-directed mutants, charge state distributions and hydrogen/deuterium exchange rates were monitored by electrospray ionization mass spectrometry. The relative stability of the mutants was observed with the order V11 insert > Y75F > wild-type = K32E > K12D = K14E > or = K52E > K14E/K32E > W67Y > P35A > I57N > G34S. (A preliminary account has been presented for mutants G34S and P35A [Jaquinod et al. (1995) Rapid Commun. Mass Spectrom. 9, 1135-1140].) This approach is shown to be a useful tool for rapid characterization of mutational effects on protein conformation.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Rhodobacter capsulatus
/
Grupo dos Citocromos c
/
Mutação
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article