Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell.
Science
; 273(5272): 242-5, 1996 Jul 12.
Article
em En
| MEDLINE
| ID: mdl-8662508
ABSTRACT
Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity. Unlike in humans, primitive adult mouse bone marrow HSCs were detected in the mCD34 low to negative fraction. Injection of a single mCD34(lo/-), c-Kit+, Sca-1(+), lineage markers negative (Lin-) cell resulted in long-term reconstitution of the lymphohematopoietic system in 21 percent of recipients. Thus, the purified HSC population should enable analysis of the self-renewal and multilineage differentiation of individual HSCs.
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Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Células-Tronco Hematopoéticas
/
Antígenos CD34
/
Hematopoese
Limite:
Animals
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article