RcsC-mediated induction of colanic acid by secretion of streptokinase in Escherichia coli K-12.
FEMS Microbiol Lett
; 139(2-3): 189-93, 1996 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-8674987
ABSTRACT
The introduction of a plasmid containing skc (streptokinase-coding gene) fused with ompA signal sequence into Escherichia coli K-12 strains, rendered the bacteria mucoid. Measurement of the synthesis of beta-galactosidase from a cps-lacZ fusion (lacZ fusion to a gene necessary for capsule synthesis) showed that the mucoid phenotype was due to induction of the capsular polysaccharide colanic acid synthesis. The introduction of a plasmid carrying skc fused with malE (gene encoding maltose-binding protein) also induced cps-lacZ expression, but intracellular expression of streptokinase in E. coli did not. The cps expression by secretion of streptokinase was diminished to the basal level in a cps-lacZ strain carrying a rcsC mutation. These results show that the secretion of streptokinase in E. coli induces colanic acid synthesis through the RcsC-dependent pathway.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Proteínas Quinases
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Estreptoquinase
/
Proteínas de Bactérias
/
Fatores de Transcrição
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Fosfoproteínas Fosfatases
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Proteínas de Escherichia coli
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Escherichia coli
/
Complexos Multienzimáticos
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article