Sterol specificity of the Saccharomyces cerevisiae ERG6 gene product expressed in Escherichia coli.
Lipids
; 31(4): 373-7, 1996 Apr.
Article
em En
| MEDLINE
| ID: mdl-8743048
ABSTRACT
The ERG6 gene from Saccharomyces cerevisiae has been functionally expressed in Escherichia coli, for the first time, yielding a protein that catalyzes the bisubstrate transfer reaction whereby the reactive methyl group from (S)-adenosyl-L-methionine is transferred stereoselectively to C-24 of the sterol side chain. The structural requirements of sterol in binding and catalysis were similar to the native protein from S. cerevisiae. Inhibition of biomethylation was observed with fecosterol and ergosterol which suggests that ergosterol may function in wild-type yeast as feedback regulator of sterol biosynthesis.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Esteróis
/
Metiltransferases
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article