Anionic properties of beta-lactam-enhancing factor on methicillin-resistant Staphylococcus aureus.
Res Microbiol
; 147(4): 279-86, 1996 May.
Article
em En
| MEDLINE
| ID: mdl-8763615
ABSTRACT
We found a Factor (factor T) in aged mixtures of tungstate and phosphate which greatly enhanced the antibacterial effects of beta-lactams upon methicillin-resistant Staphylococcus aureus (MRSA). Factor T suppressed penicillinase production and the amount of penicillin-binding protein-2' in the membrane fraction, thus sensitizing MRSA strains to beta-lactams. In addition, Factor T caused a metachromatic reaction and prolonged the blood coagulation time, indicating that it is a heparin-like polyanion. Since Factor T becomes ineffective in the presence of a polycation, a charge interaction may play an important role in the enhancing effect. One possibility is that Factor T non-specifically inhibits several anion-sensitive enzymes. Factor T inhibited several nucleotide-interacting enzymes, but not most serum enzymes.
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Base de dados:
MEDLINE
Assunto principal:
Penicilinase
/
Fosfatos
/
Staphylococcus aureus
/
Membrana Celular
/
Compostos de Tungstênio
/
Antibacterianos
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article