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Investigations on the specificity of thiophilic interaction for monoclonal antibodies of different subclasses.
Finger, U B; Brümmer, W; Knieps, E; Thömmes, J; Kula, M R.
Afiliação
  • Finger UB; Institute of Enzymetechnology, Heinrich-Heine University Düsseldorf, Jülich, Germany.
J Chromatogr B Biomed Appl ; 675(2): 197-204, 1996 Jan 26.
Article em En | MEDLINE | ID: mdl-8852706
A comparative study was carried out to investigate the influence of different mouse antibody subclasses on the chromatographic behaviour on thiophilic supports. Cell-free supernatants from different mouse-mouse hybridoma cultures in a standard medium were purified on thiophilic agarose and Fractogel EMD TA. The adsorption capacities and purification factors were monitored under optimised adsorption conditions. The different isotypes did not differ significantly regarding capacity of the thiophilic matrix, but the purity of the eluted antibody fractions was significantly lower for the IgG2a subclass compared to all other murine antibodies. A significant copurification of proteins from cell culture supernatant with antibodies of the IgG2a subclass indicated a restriction in the universal nature of thiophilic interaction.
Assuntos
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Base de dados: MEDLINE Assunto principal: Anticorpos Monoclonais / Especificidade de Anticorpos Limite: Animals Idioma: En Ano de publicação: 1996 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Anticorpos Monoclonais / Especificidade de Anticorpos Limite: Animals Idioma: En Ano de publicação: 1996 Tipo de documento: Article