Acquisition of host cell-surface-derived molecules by HIV-1.
AIDS
; 10(14): 1611-20, 1996 Dec.
Article
em En
| MEDLINE
| ID: mdl-8970680
ABSTRACT
OBJECTIVE:
To determine the acquisition of host cell-membrane-derived molecules by HIV-1 during the budding process, and to investigate whether the uptake of these molecules is cell-type-specific and selective.DESIGN:
Virions, propagated by four different cell types were analysed for the presence of adhesion molecules, glycosylphosphatidylinositol (GPI)-anchored proteins and various cell-surface markers. The pattern was compared with the phenotype of the HIV-1-infected cell.METHODS:
For phenotypic analysis of virions a two-step assay was used. In the first step, virus was captured with monoclonal antibodies (in some cases polyclonal sera) against different cell-membrane proteins. In a second step, the presence of virus was measured by determining the concentration of the virus-specific p24 core antigen. The expression of surface molecules on uninfected and HIV-1IIIB-infected cells was analysed by FACS.RESULTS:
Depending on the cell type used for virus propagation, different cell-membrane molecules were found on the virus surface reflecting the corresponding cell type. The uptake of these molecules was selective to a certain degree. No CD4 and CD87 molecules were detectable on HIV-1, although both molecules were present on uninfected and HIV-1-infected cells. CR3 and CDw108 could not be seen on uninfected cells, but wre detectable on infected cells and virions.CONCLUSIONS:
During the budding process HIV-1 acquires a variety of cell-type-specific cell-surface molecules. Certain cell-membrane molecules become upregulated during HIV-1-infection and are then found on virions, whereas other molecules remain on the cell surface and do not become incorporated.
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Base de dados:
MEDLINE
Assunto principal:
Infecções por HIV
/
Membrana Celular
/
HIV-1
/
Proteínas de Membrana
Limite:
Humans
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article