Mutational spectra in transgenic animal research: data analysis and study design based upon the mutant or mutation frequency.
Environ Mol Mutagen
; 28(4): 405-13, 1996.
Article
em En
| MEDLINE
| ID: mdl-8991070
Understanding chemically induced changes in mutational spectra can aid in deciphering mechanisms of mutagenesis. In this paper, we propose the use of statistical methods that are based upon the mutation frequency, rather than simple mutant counts which have no relationship to the mutation frequency. These methods have a number of advantages over the current standard analysis: an improved means of identifying those classes/sites of mutation which have treatment-related induction, greater sensitivity to localized differences in spectra (e.g., limited to a single base pair), one-sided tests for induction of mutations, tests of dose-response, and a framework for sample-size estimation in terms of the number of mutants to sequence. As examples, the methods are applied to data from transgenic mutation assays.
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Base de dados:
MEDLINE
Assunto principal:
Projetos de Pesquisa
/
Animais Geneticamente Modificados
/
Modelos Estatísticos
/
Modelos Genéticos
/
Mutação
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article