Measuring serotonin distribution in live cells with three-photon excitation.
Science
; 275(5299): 530-2, 1997 Jan 24.
Article
em En
| MEDLINE
| ID: mdl-8999797
ABSTRACT
Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established by 3PE cross section measurements of tryptophan and the monoamines serotonin and dopamine, circumvents the limitations imposed by photodamage, scattering, and indiscriminate background encountered in other UV microscopies. Three-dimensionally resolved images are presented along with measurements of the serotonin concentration ( approximately 50 mM) and content (up to approximately 5 x 10(8) molecules) of individual secretory granules.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Serotonina
/
Fótons
/
Grânulos Citoplasmáticos
/
Microscopia de Fluorescência
Limite:
Animals
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article