Cloning of biogenic amine receptors from moths (Bombyx mori and Heliothis virescens).
Insect Biochem Mol Biol
; 26(8-9): 817-27, 1996.
Article
em En
| MEDLINE
| ID: mdl-9014328
ABSTRACT
Based on the similarity of genes which code for guanine-nucleotide binding protein- (G-protein-) coupled receptors, cDNA clones encoding new members of the receptor family have been isolated from Bombyx mori and Heliothis virescens. The deduced protein structures exhibit highest similarity to tyramine/octopamine and serotonin receptors of Drosophila. One of the receptor clones (K50Hel) was permanently expressed in the mammalian cell line LLC-PK1. In stimulation experiments its responded to octopamine leading to an inhibition of adenylate cyclase activity in a dose-dependent manner. Pharmacological studies revealed a higher affinity for mianserin than for yohimbine suggesting, that the K50Hel clone encoded a neuronal type 3 octopamine receptor. As revealed by in situ hybridization, this receptor type is expressed in the central nervous system and antennae of moth.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Bombyx
/
Octopamina
/
Receptores de Amina Biogênica
/
Mariposas
Limite:
Animals
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article