Extension of the replicative life span of human diploid fibroblasts by inhibition of the p33ING1 candidate tumor suppressor.
Mol Cell Biol
; 17(4): 2014-9, 1997 Apr.
Article
em En
| MEDLINE
| ID: mdl-9121449
ABSTRACT
Previous studies suggest that tumor suppressors may play significant roles in blocking the growth of cells during cellular senescence. We therefore studied the potential involvement of a novel growth inhibitor and candidate tumor suppressor gene called ING1, which we have cloned recently (I. Garkavtsev, A. Kazarov, A. Gudkov, and K. Riabowol, Nat. Genet. 14415-420, 1996), in the process of cellular senescence. Our results show that the RNA and protein levels of ING1 were 8- to 10-fold higher in senescent cells than in young, proliferation-competent human diploid fibroblasts. Expression of the nuclear p33ING1 protein was regulated during the cell cycle, reaching maximal levels during DNA synthesis. Chronic expression of antisense ING1 RNA reproducibly resulted in extension of the proliferative life span of normal human fibroblasts by approximately seven population doublings.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Genes Supressores de Tumor
/
Fibroblastos
/
Inibidores do Crescimento
Limite:
Humans
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article