Dose-response effect of in vivo administration of endotoxin on polymorphonuclear leukocytes oxidative burst.
Shock
; 5(5): 357-61, 1996 May.
Article
em En
| MEDLINE
| ID: mdl-9156792
ABSTRACT
In vitro, endotoxin primes polymorphonuclear leukocytes (PMNs) to respond with a greater oxidative burst. The purpose of the present study was to investigate the in vivo effect of a wide range of single endotoxin bolus doses using a rat model. PMNs were subsequently challenged in vitro with phorbol ester to produce reactive oxygen intermediates (ROI). Flow cytometric determination of ROI production by large doses induced a decrease in ROI production by the few PMNs that remained in the circulation. By 6 h after injection, ROI production had returned to basal levels after a high dose, and was still increasing after a low dose. Neutropenia occurred immediately after endotoxin injection. After 6 h, PMN counts returned to almost normal levels with a high dose, but rebound neutrophilia occurred with a small dose. In contrast to in vitro studies, in vivo injection showed a response pattern that varied widely with dose and time of observation.
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Base de dados:
MEDLINE
Assunto principal:
Explosão Respiratória
/
Espécies Reativas de Oxigênio
/
Endotoxinas
/
Neutrófilos
Limite:
Animals
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article