Effects of gestation on enzymes controlling aldosterone synthesis in the rat adrenal.
Endocrinology
; 138(6): 2354-8, 1997 Jun.
Article
em En
| MEDLINE
| ID: mdl-9165022
ABSTRACT
In the present study, the effects of gestation on various enzymes implicated in corticosteroid synthesis were evaluated in adrenal zona glomerulosa and zona fasciculata-reticularis of the Sprague-Dawley rat. The activity and expression of cholesterol side-chain cleavage cytochrome P450, 11beta-hydroxylase cytochrome P450, and aldosterone synthase cytochrome P450 (P450aldo) were analyzed. Plasma aldosterone levels were increased significantly at 22 days gestation (n = 10) and fell below the nonpregnant levels at 18-36 h postpartum (n = 11). The activity and expression of 11beta-hydroxylase cytochrome P450 and cholesterol side-chain cleavage cytochrome P450 were not modified by gestation. P450aldo activity increased at 14 days gestation (n = 4) and returned to the prepregnancy level at 2 weeks postpartum (n = 5). As shown by Northern blot analysis (n = 3), P450aldo messenger RNA increased significantly at 22 days gestation and decreased 18-36 h postpartum. We clearly demonstrated that elevated plasma aldosterone levels during pregnancy are associated with augmented activity and messenger RNA levels of P450aldo in the zona glomerulosa.
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Base de dados:
MEDLINE
Assunto principal:
Prenhez
/
Enzima de Clivagem da Cadeia Lateral do Colesterol
/
Esteroide 11-beta-Hidroxilase
/
Córtex Suprarrenal
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Citocromo P-450 CYP11B2
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Aldosterona
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article