Long-term regulation of opioid receptors in neuroblastoma and lymphoma cell lines.
J Neuroimmunol
; 76(1-2): 145-52, 1997 Jun.
Article
em En
| MEDLINE
| ID: mdl-9184644
Long-term regulation of opioid binding was studied in the human neuroblastoma NMB and in the murine lymphoma R1.1 and R1.EGO cell lines. Binding was down-regulated following prolonged exposure to opioid agonists and up-regulated following exposure to antagonist. Down-regulation was inhibited by the metabolic blocker sodium-azide and by the protein kinase H-7. Up-regulation was blocked by the protein and mRNA synthesis blockers cycloheximide, alpha-amanitin and actinomycin D. A significant difference was found between the response of neuronal and immune cells to ethanol exposure: while opioid binding in neuroblastoma culture underwent a pronounced (75%) up-regulation, no effect of ethanol on opioid receptors in lymphoma cultures was detected. The described cell lines present an excellent experimental model to study long-term regulation of opioid receptors in the nervous and immune systems and to elucidate the biological effects of chronic use of opiates and alcohol.
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Base de dados:
MEDLINE
Assunto principal:
Receptores Opioides
/
Linfoma
/
Neuroblastoma
Limite:
Humans
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article