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Effects of calmodulin antagonists on calcium pump and cytosolic calcium level in human neutrophils.
Capuozzo, E; Verginelli, D; Crifò, C; Salerno, C.
Afiliação
  • Capuozzo E; Department of Biochemical Sciences, University of Rome La Sapienza and C.N.R. Center of Molecular Biology, Italy.
Biochim Biophys Acta ; 1357(1): 123-7, 1997 Jun 05.
Article em En | MEDLINE | ID: mdl-9202183
The concentration of cytosolic free calcium was monitored in suspensions of intact human neutrophils in phosphate-buffered saline by means of the fluorescent indicator Indo 1 trapped in the cytosol. Trifluoperazine and n-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide markedly reduced the amplitude of the transient increase in cytosolic Ca2+ triggered by CaCl2 as well as by N-formyl-methionyl-leucyl-phenylalanine. The effect of the calmodulin antagonists on the calcium burst observed upon cell activation was much more pronounced in the presence of extracellular free calcium than in EGTA-containing media; it was not inhibited by wortmannin or thapsigargin. Nevertheless, trifluoperazine and n-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide inhibited the plasma-membrane Ca2+ ATPase if added to plasma membrane-enriched fractions of neutrophils. These results suggest that calmodulin antagonists affect calcium ion influx even if they inhibit plasma membrane Ca2+ ATPase.
Assuntos
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Base de dados: MEDLINE Assunto principal: Calmodulina / Cálcio / ATPases Transportadoras de Cálcio / Citosol / Neutrófilos Limite: Humans Idioma: En Ano de publicação: 1997 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Calmodulina / Cálcio / ATPases Transportadoras de Cálcio / Citosol / Neutrófilos Limite: Humans Idioma: En Ano de publicação: 1997 Tipo de documento: Article