Structure of the 13C-enriched O-deacetylated glucuronoxylomannan of Cryptococcus neoformans serotype A determined by NMR spectroscopy.
Carbohydr Res
; 301(1-2): 33-40, 1997 Jun 11.
Article
em En
| MEDLINE
| ID: mdl-9228737
ABSTRACT
The complete assignment of 1H and 13C chemical shifts for 99% uniformly 13C-labeled O-deacetylated glucuronoxylomannan (GXM) of Cryptococcus neoformans serotype A isolate 9759-Mu-1 was accomplished by the analysis of HCCH-TOCSY and HCCH-COSY spectra. The sequence of the glycosyl residues was determined by a GHMBC experiment using 20% uniformly 13C-labeled GXM; GXM was prepared by a novel procedure that insured the virtual exclusion of adjacent 13C-labeled carbon atoms. For each residue in the GXM of 9759-Mu-1 we determined its linkage position, its anomeric configuration, and its position in the repeating sequence as follows [sequence see text]
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Cryptococcus neoformans
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article