Analysis of nitrate in biological fluids by capillary electrophoresis.
J Chromatogr A
; 781(1-2): 491-6, 1997 Sep 26.
Article
em En
| MEDLINE
| ID: mdl-9368397
ABSTRACT
Nitrite and nitrate represent the products of the final pathway of nitric oxide metabolism. These two ions were analyzed by capillary electrophoresis (CE) in serum, cerebrospinal fluid, urine and tissue homogenates by mixing the sample with acetonitrile containing NaBr as an internal standard, followed by centrifugation. The supernatant was injected hydrodynamically on a capillary 50 cm x 75 microns (I.D.) and electrophoresed at 6 kV (reversed polarity) in 1.4% sodium chloride in phosphate buffer for 13 min with detection at 214 nm. In addition to removal of the proteins, acetonitrile caused sample stacking. Urinary nitrate analysis by CE was compared to that by the enzymatic Aspergillus nitrate reductase method, with a correlation coefficient of 0.96.
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Base de dados:
MEDLINE
Assunto principal:
Líquidos Corporais
/
Eletroforese Capilar
/
Nitratos
/
Nitritos
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article