Analysis of cell kinetics after gamma-ray irradiation using an anti-BrdU monoclonal antibody.
Int J Oncol
; 12(2): 345-9, 1998 Feb.
Article
em En
| MEDLINE
| ID: mdl-9458360
ABSTRACT
The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrdU) monoclonal antibody and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied primarily by counting G2-arrested cells. By the present BrdU method, a rapid transition from G1 to S-phase was observed within 2 h of irradiation, followed by G1 block. Cells in the S-phase progressed to G2+M where they arrested, resulting in a decreased percentage of S cells (<5%). Release of G1 block occurred after 8 h, and G2+M cells returned to G1 after >18 h. The initial G1 arrest induced by irradiation was confirmed for the first time by the present BrdU-PI double staining.
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Base de dados:
MEDLINE
Assunto principal:
Ciclo Celular
/
Raios gama
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article