A study on the receptor for a mycobacteriophage : phage phlei.
Biochimie
; 58(1-2): 87-97, 1976.
Article
em En
| MEDLINE
| ID: mdl-953052
ABSTRACT
From Mycobacterium phlei, glycolipid fractions have been isolated which inactivate phage Phlei. On the basis of the characteristics of the inactivation (specificity, kinetics, requirement for Ca++) typical of the phage-host cell system, it was concluded that these fractions contain the receptor sites for phage Phlei ; this conclusion was supported by electron microscopic studies. All the active fractions contain four kinds of components fatty acids, glycerol, sugars (D-lyxose, 6-0-methyl-D-glucose, and low amounts of glucose and mannose), and water-soluble acids. These acids are isolated by degradation of the receptor fractions as oxalic and pyruvic acids. Variations of the ratio oxalic acid/pyruvic acid according to the mode of degradation and the absence of the peak characteristic of the protons of a pyruvic acid residue in the NMR spectrum, suggest that these acids might arise from the splitting of oxaloacetic acid. A tentative structure of the receptor is proposed, in many monoglycerides are linked through keto-acid to a polysaccharide core.
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Base de dados:
MEDLINE
Assunto principal:
Sítios de Ligação
/
Glicolipídeos
/
Micobacteriófagos
/
Mycobacterium
/
Mycobacterium phlei
Idioma:
En
Ano de publicação:
1976
Tipo de documento:
Article