Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells.
Biochim Biophys Acta
; 1371(1): 53-70, 1998 Apr 22.
Article
em En
| MEDLINE
| ID: mdl-9565656
ABSTRACT
Cationic lipids are considered to be capable of efficiently and safely mediating DNA transfer into cells, although expression is transient. A new family of cationic lipids, called phosphonolipids, has been developed, with the relationship between the hydrophobic domain of the lipid molecules and the significant enhancement of transduction efficiency in a non-adherent cell line characterised in the present study. The kinetics of transfection efficiency were also investigated. Our results demonstrate that the peak of the transient expression of these reporter genes mediated by cationic lipids occurred within 3 to 14 days, depending on the aliphatic chain length of the complex used and on its formulation in the presence or absence of DOPE. Furthermore, the kinetics of transgene expression were found to differ in adherent and non-adherent cells. These results were obtained using three different techniques CPRG, luminescence, and FACS-gal, and were in agreement with electron microscopy studies. We thus hypothesized that the plasma membrane composition of cells could affect the efficiency of transfection with cationic lipids. Our results suggest that phosphonolipids constitute a promising class of compounds for gene transfer protocols, and that galenic optimization should improve and modify the transfection efficiency of these DNA-lipid complexes.
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Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Transfecção
/
Transgenes
/
Sistema Hematopoético
Limite:
Humans
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article