Direct evidence of methylglucose lipopolysaccharides/palmitoyl-CoA noncovalent complexes by capillary zone electrophoresis-electrospray/mass spectrometry.
Anal Chem
; 70(9): 1853-8, 1998 May 01.
Article
em En
| MEDLINE
| ID: mdl-9599585
ABSTRACT
Mycobacterial methylglucose lipopolysaccharides (MGLPs) play an important regulatory role in the biosynthesis of long-chain fatty acids by forming complexes with neosynthesized acyl-CoA fatty acid derivatives. The MGLPs from Mycobacterium smegmatis were purified by high-performance anion-exchange chromatography and characterized by LSIMS and CE/ESI-MS. We investigated their interaction with palmitoyl-CoA using capillary zone electrophoresis with both direct and indirect UV detection. In the latter mode, the signal of the UV-transparent MGLPs decreased upon addition of increasing amounts of palmitoyl-CoA; while using direct UV detection, the addition of palmitoyl-CoA to the MGLPs revealed characteristic profiles. The major peak was assigned to the noncovalent MGLP/palmitoyl-CoA complex on the basis of its electrophoretic mobility. The abundance of the complex was found to increase until the MGLP/palmitoyl-CoA molar ratio reached a 1/1 stoichiometry. The existence of and the stoichiometry of this complex were assessed by CE/ESI mass spectrum analysis, showing pseudomolecular ions of the MGLP/palmitoyl-CoA complex. These results confirm that CE/ESI-MS is a powerful tool to characterize noncovalent molecular association.
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Base de dados:
MEDLINE
Assunto principal:
Palmitoil Coenzima A
/
Lipopolissacarídeos
/
Metilglucosídeos
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article