The intron 5-inserted form of rat erythropoietin receptor is expressed as a membrane-bound form.
Biochim Biophys Acta
; 1403(2): 169-78, 1998 Jun 22.
Article
em En
| MEDLINE
| ID: mdl-9630610
ABSTRACT
The cDNA encoding an intron 5-inserted form of the erythropoietin receptor (I5Epo-R) has been cloned from rat. DNA sequence analysis reveals that the insertion of intron 5, which consists of 79 bp, causes a shift in reading frame and results in termination in the region of exon 7. The deduced amino acid sequence is composed of 316 amino acid residues, which is a molecular weight of 34220. To study the function of rat I5Epo-R, we established a Chinese hamster ovary cell line expressing rat I5Epo-R. Western blot analysis and binding studies with 125I-recombinant human erythropoietin showed that the transfected cells expressed rat I5Epo-R with a molecular size of 36 kDa as a membrane-bound form, but not as a soluble form, and had a single class of binding sites with a Kd of 700 pM.
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Base de dados:
MEDLINE
Assunto principal:
Íntrons
/
Receptores da Eritropoetina
/
Processamento Alternativo
/
Proteínas de Membrana
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article