Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves.
Gene
; 212(2): 279-86, 1998 Jun 08.
Article
em En
| MEDLINE
| ID: mdl-9678973
ABSTRACT
The import of nuclear proteins into nuclei begins with recognition of nuclear localization signal-harboring proteins and binding to a nuclear pore targeting complex. A cDNA for an importin-alpha protein, a subunit of the complex, was isolated from rice plants. The amino acid sequence deduced from the nucleotide sequence of the cDNA exhibited a high homology to those of importin-alpha proteins from many organisms such as Arabidopsis thaliana, Saccharomyces cerevisiae, human, mouse, Xenopus laevis and Drosophila melanogaster. Down-regulation of the transcription by light was shown in the leaves of light- and dark-grown seedlings by RNA blot analysis. The down-regulation was specific to leaves, whereas no light effect was observed in root tissues or calli, in which higher levels of the transcript were detected.
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Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Proteínas Nucleares
/
Regulação para Baixo
/
Genes de Plantas
/
DNA Complementar
/
Folhas de Planta
/
Luz
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article