Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains.
Science
; 281(5377): 666-9, 1998 Jul 31.
Article
em En
| MEDLINE
| ID: mdl-9685252
ABSTRACT
It was recently demonstrated that peptide bond formation can occur using an Escherichia coli naked 23S ribosomal RNA without any of the ribosomal proteins. Here, the six domains of the 23S ribosomal RNA were individually synthesized and shown to be capable, when complexed together, of stimulating the reaction. Omission and addition experiments indicated that the activity could be reconstituted solely by domain V at a concentration 10 times higher than that of the intact 23S ribosomal RNA, whereas domain VI could enhance the activity in trans. These findings suggest that fragments of an RNA molecule have the ability to associate into a functional whole.
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Base de dados:
MEDLINE
Assunto principal:
Biossíntese Peptídica
/
RNA Ribossômico 23S
/
Aminoacil-RNA de Transferência
/
Peptidil Transferases
/
Escherichia coli
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article