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Biochemical and genetic analysis of lambdaW, the newly isolated lambdoid phage.
Wróbel, B; Srutkowska, S; Wegrzyn, G.
Afiliação
  • Wróbel B; Department of Molecular Biology, University of Gdansk, Poland.
Acta Biochim Pol ; 45(1): 251-9, 1998.
Article em En | MEDLINE | ID: mdl-9701518
ABSTRACT
Otherwise isogenic Escherichia coli CP78 (relA+) and CP79 (relA-) strains are commonly used in studies on the stringent control, the bacterial response to amino acid starvation. We found that these strains are lysogenic for a phage which is spontaneously induced with a low frequency, producing virions able to infect other E. coli strains. Genetic studies, restriction analysis of the phage DNA genome, and electron microscopy revealed that this phage is very similar to, but not identical with, bacteriophage lambda. We called the newly isolated phage lambdaW, and found that most of CP78/CP79 ancestor strains are lysogenic for this phage.
Assuntos
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Base de dados: MEDLINE Assunto principal: Bacteriófago lambda / Escherichia coli / Lisogenia Idioma: En Ano de publicação: 1998 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Bacteriófago lambda / Escherichia coli / Lisogenia Idioma: En Ano de publicação: 1998 Tipo de documento: Article