Simian virus 40 footprints in normal human tissues, brain and bone tumours of different histotypes.
Dev Biol Stand
; 94: 55-66, 1998.
Article
em En
| MEDLINE
| ID: mdl-9776226
ABSTRACT
SV40 footprints were investigated by PCR in normal human tissues and tumours of different histotypes, followed by Southern blot hybridization with a specific internal oligoprobe for SV40 DNA. Specific SV40 amplification products were detected at high prevalence in primary human brain tumours 83% of choroid plexus papillomas, 75% ependymomas, 47% astrocytomas and 37% glioblastomas. SV40 footprints were also revealed in primary bone tumours 35% osteosarcomas and Ewing's tumours. Positive normal tissue samples ranged from 45% of sperm fluids to 8% of brain tissue. Normal bone tissue specimens were SV40 negative. These results indicate that SV40 is associated with human brain and bone neoplasms, whereas normal bone and brain tissues were either SV40 negative or positive at low grade. SV40 footprints were found in other normal samples such as PBC, B- and T-lymphocytes and sperm fluids, indicating that SV40 is latent in these cells. Therefore, these cells may be vectors of SV40 in other host tissues and may spread SV40 infection by blood transfusion and sexual transmission in the human population.
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Base de dados:
MEDLINE
Assunto principal:
Infecções Tumorais por Vírus
/
Osso e Ossos
/
Encéfalo
/
DNA Viral
/
Vírus 40 dos Símios
/
Infecções por Papillomavirus
/
Neoplasias
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article