Comparison of polyclonal, monoclonal and protein G peroxidase conjugates in an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis in sheep.
Vet Rec
; 143(14): 390-4, 1998 Oct 03.
Article
em En
| MEDLINE
| ID: mdl-9802197
The sensitivities of three commercial peroxidase conjugates (polyclonal anti-sheep IgG, recombinant protein G and a monoclonal anti-ruminant IgG1) in an enzyme-linked immunosorbent assay for Brucella ovis were evaluated. The monoclonal and protein G conjugates reduced, but did not totally remove, the characteristic background reactivity observed with the sera from brucella-free sheep. The protein G conjugate provided the best sensitivity, similar to that obtained with the classical gel diffusion test. Both tests were highly specific when testing sera from brucella-free animals but detected as positive a large proportion of sera from both Brucella melitensis-infected and B melitensis Rev 1-vaccinated sheep.
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Base de dados:
MEDLINE
Assunto principal:
Doenças dos Ovinos
/
Brucella
/
Brucelose
/
Imunoglobulina G
/
Ensaio de Imunoadsorção Enzimática
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article