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Detection of IgG rheumatoid factor by concanavalin A treatment and complement fixation with IgG rheumatoid factor.
Ann Rheum Dis ; 35(3): 240-5, 1976 Jun.
Article em En | MEDLINE | ID: mdl-984904
Concanavalin A (Con A) froms precipitates with carbohydrate-rich protein such as IgM, IgD, IgE, and IgA. Since IgG contains little carbohydrate and does not react with Con A, the activity of IgG-rheumatoid factor (RF) can be measured in the supernate of the Con A-treated serum. When the latex fixation test (LFT) and the sensitized sheep cell agglutination test (SSCA) were perfromed in the supernate for the detection of IgG-RF, LFT was positive in 32-1% of sera, out of 137 sera originally positive for LFT, and SSCA was positive in 18-5% of sera, out of 119 sera originally positive for SSCA. IgG-RF exhibited lower complement fixing ability than IgM-RF and correlated with agglutination titres of IgG-RF, while the CH50 of the original serum did not correlate with haemolytic activities of either IgM-RF or IgG-RF.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Artrite Reumatoide / Fator Reumatoide / Imunoglobulina G / Concanavalina A Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 1976 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Artrite Reumatoide / Fator Reumatoide / Imunoglobulina G / Concanavalina A Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 1976 Tipo de documento: Article