The Lck binding domain of herpesvirus saimiri tip-484 constitutively activates Lck and STAT3 in T cells.
J Virol
; 73(2): 1689-94, 1999 Feb.
Article
em En
| MEDLINE
| ID: mdl-9882381
Constitutive activation of signal transducers and activators of transcription (STATs) has been associated with oncogenesis. Previously, a protein required for T-cell transformation by the DNA tumor virus herpesvirus saimiri (HVS) strain 484, designated tyrosine kinase-interacting protein (Tip-484), was shown to interact with and dramatically upregulate the activity of the STATs in an Lck-dependent manner. The minimal region of Tip-484 responsible for binding Lck was defined as a 10-residue C-terminal Src-related kinase homology domain, an 18-amino-acid spacer, and a 10-residue potential SH3 binding domain. This region is termed the LBD (for Lck binding domain). The present data show that only the LBD of Tip-484 is needed to activate Lck in vitro and in vivo. Finally, the LBD was shown to form a complex with STAT3 in vitro, and expression of the LBD in T cells led to STAT3 activation equal to that of full-length Tip-484. These studies demonstrate that the 48-amino-acid LBD of Tip-484 can perform as effectively as the full-length protein in vitro and in vivo.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Proteínas Virais
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Transativadores
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Herpesvirus Saimiriíneo 2
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Proteína Tirosina Quinase p56(lck) Linfócito-Específica
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Proteínas de Ligação a DNA
Limite:
Humans
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article