Selection and immunomodulatory evaluation of lactic acid bacteria suitable for use as canine probiotics / 대한수의학회지
Korean Journal of Veterinary Research
; : 81-88, 2015.
Article
em Ko
| WPRIM
| ID: wpr-114949
Biblioteca responsável:
WPRO
ABSTRACT
This study was conducted to isolate lactic acid bacteria (LAB) from dog intestine and identify potential probiotic strains for canine use. One hundred and one LAB were isolated from feces of 20 healthy dogs. Acid, bile, and heat resistance along with adherence to Caco-2 cells and antimicrobial activity against pathogens were examined. To analyze immunomodulative effects, the production of nitric oxide (NO), TNF-alpha, and IL-1beta was measured using RAW 264.7 macrophages. Additionally, RAW BLUE cells were used to evaluate nuclear factor-kappaB (NF-kappaB) generation. Ultimately, three strains were selected as canine probiotics and identified as Lactobacillus reuteri L10, Enterococcus faecium S33, and Bifidobacterium longum B3 by 16S rRNA sequence analysis. The L10 and S33 strains showed tolerance to pH 2.5 for 2 h, 1.0% Oxgall for 2 h, and 60degrees C for 5 min. These strains also had strong antimicrobial activity against Escherichia coli KCTC 1682, Salmonella Enteritidis KCCM 12021, Staphylococcus aureus KCTC 1621, and Listeria monocytogenes KCTC 3569. All three strains exerted better immunomodulatory effects than Lactobacillus rhamnosus GG (LGG), a well-known commercial immunomodulatory strain, based on NO, NF-kappaB, IL-1beta, and TNF-alpha production. These results suggested that the three selected strains could serve as canine probiotics.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Salmonella enteritidis
/
Staphylococcus aureus
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Sulfaleno
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Bactérias
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Bifidobacterium
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Bile
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NF-kappa B
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Fator de Necrose Tumoral alfa
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Enterococcus faecium
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Análise de Sequência
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
Ko
Ano de publicação:
2015
Tipo de documento:
Article