Scanning Electron Microscopic Observations of Sequential Alterations of Type 1 HSV Keratitis in Rabbits
Journal of the Korean Ophthalmological Society
; : 1174-1180, 2004.
Article
em Ko
| WPRIM
| ID: wpr-125074
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: This study aimed to investigate the microscopic findings of characteristic epithelial herpes simplex keratitis (HSK) and the changes of those findings over time. METHODS: Sixteen rabbits were inoculated with the Kos-stain of herpes simplex virus (HSV) type 1 in both eyes. Two rabbits each were enucleated on the 2nd, 3rd, 4th, 5th, 6th, 7th, and 9th day after viral inoculation and were observed with a scanning electron microscope (S-2380n, Hitachi, Japan). RESULTS: Corneas showed dendritic epithelial desquamation, changes in epithelial microvilli, and changes in intercellular adhesion. With progression, the basement membrane and basal cells were exposed, intercellular spaces were broadened, and the adhesion of basal cells to basement membrane and the intercellular adhesion were loosened. Later, infiltrations of some inflammatory cells were found. Until the last day there was no destruction of basement membrane or an exposure of stroma. CONCLUSIONS: Though it was impossible to follow up a lesion throughout the experiment because of the necessity of enucleation, and in addition, the findings could not be generalized to all the strains, this study provides a better understanding of HSK. Further studies on various manifestations of HSK from other strains of HSV are needed in the future.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Membrana Basal
/
Microscopia Eletrônica de Varredura
/
Seguimentos
/
Ceratite Herpética
/
Simplexvirus
/
Córnea
/
Espaço Extracelular
/
Ceratite
/
Microvilosidades
Tipo de estudo:
Observational_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
Ko
Ano de publicação:
2004
Tipo de documento:
Article