The Regulation of MMP-2 and -14 Expressions by TGF-beta in Lens Epithelial Cells
Journal of the Korean Ophthalmological Society
; : 1110-1116, 2006.
Article
em Ko
| WPRIM
| ID: wpr-161313
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: TGF-beta is a key regulator of epithelial-mesenchymal transition. Among the TGF-beta responses, cell migration is closely associated with the expression of matrix metalloproteinases (MMPs). Therefore, we determined which MMPs are regulated by TGF-beta and examined the TGF-beta signaling involved in this event, focusing on Src family tyrosine kinases (SFKs) METHODS: First we examined the expression of MMPs in rat lens explant culture treated with TGF-beta and LECs attached to the anterior capsules of patients with nuclear (N), anterior polar (AP) cataracts using RT-PCR and immunofluorescence staining. It was examined whether the expression of MMPs is regulated by SFKs. RESULTS: The study using RT-PCR and immunofluorescence staining showed the expression of MMP-2 and -14 in explants and the expression of MMP-14 LECs of AP cataracts. The expression of MMP-2 and -14 was blocked by PP2 in explants. Furthermore, the activated form of SFKs was observed in LECs of AP cataracts by immunofluorescence staining. CONCLUSIONS: We suggest a novel role of SFKs signaling in the expression of MMP-14 induced by TGF-beta.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Catarata
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Cápsulas
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Movimento Celular
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Fator de Crescimento Transformador beta
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Imunofluorescência
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Quinases da Família src
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Metaloproteinases da Matriz
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Células Epiteliais
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Transição Epitelial-Mesenquimal
Limite:
Animals
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Humans
Idioma:
Ko
Ano de publicação:
2006
Tipo de documento:
Article