Antifungal Susceptibility Testing of Candida Species Using ATB FUNGUS 2 / 대한피부과학회지

ABSTRACT

BACKGROUND: Because of recently increasing reports of resistance of Candida (C.) species to antifungal agents, it is necessary to perform antifungal susceptibility testing. ATB FUNGUS 2 (bioMerieux, France) is an easy-to-perform in-vitro antifungal susceptibility test. OBJECTIVE: The purpose of the study was to investigate the minimal inhibitory concentration (MIC) of antifungal agents against Candida species isolated from skin lesion using ATB FUNGUS 2. METHODS: Eighty two clinical isolates of Candida species (including 56 C. albicans, 14 C. parapsilosis, 7 C. guilliermondii, 5 C. glabrata) were testeding ATB FUNGUS 2. MICs against itraconazole (ICZ), fluconazole (FCZ), flucytosine (FC) and amphotericin B (AMP) were read after 24 hours incubation. RESULTS: MIC of ICZ was or =2.0microgram/ml on four strains (3 C. albicans, 1 C. glabrata) and 0.25~0.5microgram/ml on five strains (1 C. parapsilosis, 3 C. guilliermondii, 1 C. glabrata). MIC of FCZ was 128microgram/ml on 3 C. albicans and 16microgram/ml on 1 C. parapsilosis. MIC of FC was 64microgram/ml on 6 C. albicans. MIC of AMP was all (100.0%) <0.5microgram/ml. CONCLUSION: ATB FUNGUS 2 represented a simple and valuable method for antifungal susceptibility testing of Candida species.