A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
The Korean Journal of Parasitology
; : 503-510, 2013.
Article
em En
| WPRIM
| ID: wpr-189491
Biblioteca responsável:
WPRO
ABSTRACT
Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Fatores de Tempo
/
Toxoplasma
/
Uganda
/
Proteínas Recombinantes de Fusão
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Testes Sorológicos
/
Anticorpos Antiprotozoários
/
Dados de Sequência Molecular
/
Proteínas de Protozoários
/
Toxoplasmose
/
Reprodutibilidade dos Testes
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
País como assunto:
Africa
/
Asia
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article