Mutation of the Chk1 Gene in Gastric Cancers with Microsatellite Instability
Journal of the Korean Gastric Cancer Association
; : 260-265, 2005.
Article
em Ko
| WPRIM
| ID: wpr-189860
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: The protein kinase Chk1 is required for cell cycle arrest in response to DNA damage and is shown to play an important role in the G2/M checkpoint. The aim of this study was to investigate the relationship between microsatellite instability and frameshift mutation of the Chk1 gene in gastric cancers. MATERIALS AND METHODS: The microsatellite instability was analyzed in 95 primary gastric carcinomas by using microdissection and 6 microsatellite markers. We also performed single strand conformational polymorphism and sequencing to detect frameshift mutation of the Chk1 gene. RESULTS: We found positive microsatellite instability in 19 (20%) of the 95 gastric cancers, 13 high- and 6 low-frequency microsatellite instability cases. The frameshift mutation of Chk1, which resulted in a truncated Chk1 protein, was detected in two high-frequency microsatellite instability cases. CONCLUSION: These data suggest that the microsatellite instability may contribute to the development of gastric carcinomas through inactivation of Chk1.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Proteínas Quinases
/
Neoplasias Gástricas
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Dano ao DNA
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Ciclo Celular
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Mutação da Fase de Leitura
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Repetições de Microssatélites
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Microdissecção
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Instabilidade de Microssatélites
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Pontos de Checagem do Ciclo Celular
Idioma:
Ko
Ano de publicação:
2005
Tipo de documento:
Article