Immunohistochemical Study for the Syndecan-2 in Cutaneous Melanoma and Pigmented Nevus / 대한피부과학회지
Korean Journal of Dermatology
; : 776-781, 2013.
Article
em Ko
| WPRIM
| ID: wpr-204077
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Melanoma is a malignant neoplasm originating from melanocytes. It has been recently suggested that syndecan-2 may contribute to the aggressive phenotype and metastatic potential of melanoma in cell line studies. However, there is no quantitative analysis of syndecan-2 expression using human melanoma tissue. OBJECTIVE: This study aimed to examine the specific expression of syndecan-2 in human melanoma tissue. METHODS: A total of 35 sections of formalin-fixed, paraffin-embedded tissues were investigated for syndecan-2 expression using immunohistochemical staining. Also, a total of 6 tissues and two kinds of cell lines were analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) and western blot. RESULTS: Immunohistochemical staining of 23 cases of melanoma tissue was done, and in 5 cases (21.7%), strong expression of syndecan-2 was seen. Also, syndecan-2 was detected in human melanoma tissue and MNT-1 melanoma cells by RT-PCR and western blot analysis. CONCLUSION: We suggest that syndecan-2 expression is increased in melanoma compared to nevus. The results of this study may help to explain the clinical features of melanoma and syndecan-2.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fenótipo
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Linhagem Celular
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Western Blotting
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Reação em Cadeia da Polimerase
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Sindecana-2
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Melanócitos
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Melanoma
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Nevo
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Nevo Pigmentado
Limite:
Humans
Idioma:
Ko
Ano de publicação:
2013
Tipo de documento:
Article