Establishment of cell free conversion system with biotin-labelled recombinant PrPsen expressed in E. coli / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 214-218, 2006.
Article
em En
| WPRIM
| ID: wpr-229699
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To report a protocol using biotin-labelled PrP protein in cell free conversion assay instead of isotope.</p><p><b>METHODS</b>A hamster PrP protein (HaPrP) was expressed in E. coli and purified with HIS-tag affinity chromatograph. After being labelled with biotin, HaPrP was mixed with PrPSc preparation from scrapie strain 263K.</p><p><b>RESULTS</b>Protease-resistant bands were detected after four-day incubation.</p><p><b>CONCLUSION</b>The new conversion model provides a reliable, easily handling, and environment-friendly method for studies of prion and transmissible spongiform encephalopathies.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
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Peptídeo Hidrolases
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Coloração e Rotulagem
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Biotina
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Proteínas Recombinantes
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Sistema Livre de Células
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Regulação da Expressão Gênica
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Western Blotting
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Cromatografia de Afinidade
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Doenças Priônicas
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article