Intranasal immunization with a flagellin-adjuvanted peptide anticancer vaccine prevents tumor development by enhancing specific cytotoxic T lymphocyte response in a mouse model
Clinical and Experimental Vaccine Research
; : 128-134, 2013.
Article
em En
| WPRIM
| ID: wpr-23176
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
Human papillomavirus (HPV) is a significant cause of cervical cancer-related deaths worldwide. Because HPV is a sexually transmitted mucosal pathogen, enhancement of antigen-specific mucosal immune response likely serves good strategy for vaccination. However, mucosal vaccines generally do not induce strong enough immune responses. Previously we proved that a bacterial flagellin, Vibrio vulnificus FlaB, induce strong antigen-specific immune responses by stimulating the Toll-like receptor 5. In this study, we tested whether FlaB could serve as an effective mucosal adjuvant for a peptide-based HPV preventive cancer vaccine. MATERIALS ANDMETHODS:
Mice were intranasally administered with a mixture of FlaB and E6/E7 protective peptides in 5-day interval for a total of two times. Five-days after the last vaccination, cellular immune responses of the vaccinated mice were analyzed. Tumor growth was also observed after a subcutaneous implantation of TC-1 cells bearing E6/E7 antigens.RESULTS:
Intranasal administration of the E6/E7 peptide mixture with FlaB elicited a strong antigen-specific cytotoxic T lymphocyte activity and antigen-specific interferon-gamma production from splenocytes and cervical lymph node cells. Furthermore, FlaB, as a mucosal adjuvant, conferred an excellent protection against TC-1 tumor challenge with high survival rates in E6/E7 immunized animals.CONCLUSION:
These results indicate that FlaB can be a promising mucosal adjuvant for nasal HPV vaccine development.Palavras-chave
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Peptídeos
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Ursidae
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Administração Intranasal
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Vacinas
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Linfócitos
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Taxa de Sobrevida
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Imunização
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Interferon gama
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Vacinação
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Imunidade nas Mucosas
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article