Genomics analysis of miRNA in colon cancer tissues / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 1130-1132, 2014.
Article
em Zh
| WPRIM
| ID: wpr-235001
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the differential expression of miRNAs in colon cancer tissues and matched tumor adjacent tissues.</p><p><b>METHODS</b>Differential expression of microRNAs in twenty paired human colon cancer tissues and adjacent tissues were detected by QPCR. miRNAs with significantly differential expression (fold change >2.4 and P<0.01) were screened to analyze their accumulation by Cluster analysis. Thus correlation of miRNA and other colon cancer-associated proteins was examined.</p><p><b>RESULTS</b>Expressions of 17 miRNAs were significantly reduced in colon cancer tissues. Cluster analysis showed that miR763-3, miR451 and miR99a had similar expression. Plasma CK20 level was negatively correlated with miR100 (r=-0.948), miR152a-5p (r=-0.948), miR125b (r=-0.949), miR145 (r=-0.949) and miR145*(r=-0.949) (all P<0.05).</p><p><b>CONCLUSION</b>miR145 and other 16 miRNAs may be used as diagnostic molecular markers of colon cancer, and miR100, miR125a-5p, miR125b, miR145 and miR145* may become the molecular markers of colon cancer lymph node metastasis.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Análise por Conglomerados
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Regulação Neoplásica da Expressão Gênica
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Neoplasias do Colo
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Perfilação da Expressão Gênica
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Genômica
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MicroRNAs
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Genética
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Metástase Linfática
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2014
Tipo de documento:
Article