Effect of 1,25-(OH)2D3 on expression of TIM-4 in the lungs of asthmatic mice / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 67-70, 2013.
Article
em Zh
| WPRIM
| ID: wpr-236872
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a mouse model of asthmatic airway remodeling and investigate the effects of 1,25-(OH)2D3 on airway structure and T cell immunoglobulin mucin protein-4 (TIM-4) expression in asthmatic mice.</p><p><b>METHODS</b>Thirty female mice (BALB/c strain) were randomly divided into control, asthma and 1,25-(OH)2D3 intervention groups. An asthmatic mouse model was induced using ovalbumin. Lung tissue of the mice was collected, mRNA expression of TIM-4 was evaluated by RT-PCR and airway remodeling and protein expression of TIM-4 were observed by hematoxylineosin staining and immunohistochemistry.</p><p><b>RESULTS</b>Typical airway remodeling was found in the asthma group, and TIM-4 expression in this group was significantly higher than in the control group (105±9 vs 42±5; P<0.05). Compared with the asthma group, the 1,25-(OH)2D3 intervention group showed improvement in airway remodeling and a decrease in TIM-4 expression (78±6) (P<0.05).</p><p><b>CONCLUSIONS</b>TIM-4 may be involved in the airway remodeling of mice. As a new type of immunoregulator, 1,25-(OH)2D3 can downregulate expression of TIM-4 in the lungs and improve airway remodeling in asthmatic mice.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Fisiologia
/
Asma
/
Calcitriol
/
RNA Mensageiro
/
Regulação da Expressão Gênica
/
Remodelação das Vias Aéreas
/
Genética
/
Pulmão
/
Proteínas de Membrana
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article