Pharmacokinetic study on spinosin in rat plasma after oral administration of suanzaoren extract at a single dose / 药学学报
Yao Xue Xue Bao
; (12): 448-450, 2003.
Article
em En
| WPRIM
| ID: wpr-251064
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To study the pharmacokinetics of spinosin in rat plasma after oral administration of Suanzaoren extract using sulfamethoxazole (SMZ) as internal standard by RP-HPLC method.</p><p><b>METHODS</b>Plasma samples were deproteined with acetonitrile, followed by evaporation of the acetonitrile to dryness. The residual was then resolved in mobile phase and HPLC separation was achieved on a Hypersil C18 (5 microns, 200 mm x 4.6 mm ID) column at 35 degrees C. The mobile phase consisted of acetonitrile-water-acetic acid (15:85:1) at a flow rate of 0.7 mL.min-1. The UV detection wavelength was set at 334 nm.</p><p><b>RESULTS</b>The calibration curve was shown to be linear over the range from 18.1 to 903.5 micrograms.L-1 (r2 > or = 0.995). Mean recovery was 94.5%. Within-day and between-day precisions RSD were less than 9.0%. The limit of quantitation was 18.1 micrograms.L-1. The plasma spinosin was stable at -20 degrees C.</p><p><b>CONCLUSION</b>The simple, sensitive and accurate HPLC method developed has been applied to determine the pharmacokinetics of spinosin in rat plasma after having taken Suanzaoren extract at a single dose.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plantas Medicinais
/
Sementes
/
Flavonoides
/
Sangue
/
Medicamentos de Ervas Chinesas
/
Farmacocinética
/
Química
/
Administração Oral
/
Cromatografia Líquida de Alta Pressão
/
Ratos Wistar
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article