Determination of guanfu base A hydrochloride in plasma by LC-MS method and its pharmacokinetics in dogs / 药学学报
Acta Pharmaceutica Sinica
; (12): 551-554, 2002.
Article
em Zh
| WPRIM
| ID: wpr-251103
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To establish an analytical method for determination of guanfu base A (GFA) concentration in plasma and to study its pharmacokinetic profile in dogs.</p><p><b>METHODS</b>Six dogs were given a 7.56 mg.kg-1 dose intravenously. Blood samples were collected at various time-points after drug administration. Analytical method based on liquid chromatography-mass spectrometry (LC-MS) was established to determine the plasma concentration of GFA. Pharmacokinetic evaluation was carried out using the 3P87 program.</p><p><b>RESULTS</b>The calibration curves were linear over the concentration range from 0.42 microgram.mL-1 to 21.2 micrograms.mL-1 (gamma = 0.9994). The intra-day and inter-day precisions were generally good (< 15%) at low, medium and high concentrations. The overall recovery of the analytes was more than 80%. Six dogs were given an i.v. dose of 7.56 mg.kg-1 of GFA hydrochloride, an open three compartment model best described the concentration-time profiles for GFA. The half-lives for the rapid and slow distribution phase and terminal elimination phase (T1/2 pi, T1/2 alpha and T1/2 beta) were 0.07 h, 1.5 h, and 13.5 h, respectively. The total area under the plasma concentration-time curve (AUC), the volume of the central compartment (Vc), and plasma clearance (CLs) were 61.43 micrograms.h.mL-1, 0.37 L.kg-1 and 0.14 L.kg-1.h-1, respectively.</p><p><b>CONCLUSION</b>The analytical method was shown to be sensitive, specific, rapid and reproducible, and was suitable for pharmacokinetic studies of GFA.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plantas Medicinais
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Sangue
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Farmacocinética
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Taxa de Depuração Metabólica
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Química
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Cromatografia Líquida
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Área Sob a Curva
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Aconitum
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Alcaloides
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Compostos Heterocíclicos de 4 ou mais Anéis
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2002
Tipo de documento:
Article