PTEN negatively regulates isoproterenol-induced cardiac hypertrophy and effects of captopril on PTEN expression / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 738-742, 2005.
Article
em Zh
| WPRIM
| ID: wpr-253074
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the negative regulation role of PTEN in isoproterenol-induced cardiac hypertrophy by testing the expression of PTEN mRNA and protein and to explore the effects of captopril (Cap) on PTEN expression.</p><p><b>METHODS</b>Twenty four rats were randomly divided into three groups: control group, ISO group, and ISO+Cap group. The following parameters were examined:body weight (BW), heart weight (HW), left ventricular weight (LVW), left ventricular end-diastolic pressure (LVEDP), left ventricular end-systolic pressure (LVESP) and +/- dp/dt(max). The ratio of HW/BW and LVW/BW was calculated. PTEN mRNA and protein were tested by RT-PCR and Western blot, respectively.</p><p><b>RESULTS</b>(1) Compared with the control group, the ratio of HW/BW and LVW/BW, LVEDP and LVESP were all increased in ISO group and ISO+Cap group (P < 0.05), but +/- dp/dt(max) was decreased (P < 0.05); (2) compared with the ISO group, the ratio of HW/BW and LVW/BW, LVEDP, LVESP were all decreased in ISO+Cap group (P < 0.05), but +/- dp/dt(max) was increased (P < 0.05); (3) compared with the control group, PTEN mRNA and protein were up-regulated in ISO group and ISO+Cap group; (4) compared with the ISO group, PTEN mRNA and protein were up-regulated in ISO+Cap group.</p><p><b>CONCLUSIONS</b>PTEN mRNA and protein are up-regulated in isoproterenol-induced cardiac hypertrophy. Captopril can up-regulate PTEN expression in cardiac hypertrophy. There is a negative regulative mechanism in cardiac hypertrophy process, in which PTEN is probably an endogenous negative regulator of cardiac hypertrophy.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
RNA Mensageiro
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Captopril
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Regulação da Expressão Gênica
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Ratos Wistar
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Cardiomegalia
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Usos Terapêuticos
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Modelos Animais de Doenças
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Tratamento Farmacológico
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PTEN Fosfo-Hidrolase
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Genética
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2005
Tipo de documento:
Article